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Firefly luciferin
Firefly luciferin is the luciferin, or light-emitting compound, found in many firefly (Lampyridae) species. It is the substrate of luciferase (EC 1.13.12.7), which is responsible for the characteristic yellow light emission from many firefly species.
As with all other luciferins, oxygen is required to elicit light; however, it has also been found that adenosine triphosphate (ATP) and magnesium are required for light emission.
==History==
Much of the early work on the chemistry of the firefly luminescence was done in the lab of William D. McElroy at Johns Hopkins University. The luciferin was first isolated and purified in 1949, though it would be several years until a procedure was developed to crystallize the compound in high yield. This, along with the synthesis and structure elucidation, was accomplished by Dr. Emil H. White at the Johns Hopkins University, Department of Chemistry. The procedure was an acid-base extraction, given the carboxylic acid group on the luciferin. The luciferin could be effectively extracted using ethyl acetate at low pH from powder of approximately 15,000 firefly lanterns. The structure was later confirmed by combined use of infrared spectroscopy, UV-vis spectroscopy and synthetic methods to degrade the compound into identifiable fragments.
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